Liver Triculture MPS

Multicellular human liver microphysiological system with hepatocytes, stellate cells, and endothelial cells for stromal and vascular modulation studies

Platform Architecture

The Liver Triculture MPS is built on the modular NANOSTACKS™ platform, incorporating primary human hepatocytes, hepatic stellate cells, and liver endothelial cells in physically separated compartments for investigation of stromal and vascular modulation.

Unlike monoculture systems, this configuration maintains hepatocyte–stromal–endothelial interactions to capture microenvironment-driven drug responses and fibrosis-linked mechanistic pathways during chronic exposure.

Each layer represents a discrete, permeable cellular compartment.

Validated Performance

The Liver Triculture MPS demonstrates sustained hepatocyte metabolic function under endothelial and stellate cell cross-talk, confirming hepatocyte-intrinsic viability and performance in a multicellular microenvironment.

Representative validation data

Albumin secretion over time in Liver Triculture MPS

Representative albumin secretion over extended culture periods demonstrates sustained hepatocyte metabolic competence in the presence of supporting stromal and endothelial cells, validating the baseline performance of the triculture configuration.

Key Model Capabilities

≥28-day functional stability
Sustained hepatocyte metabolic function
Stromal–hepatocyte cross-talk
Enhanced fibrosis-relevant responses

This model is optimized for stromal and vascular modulation studies and does not include immune cells; immune-hepatic interactions are addressed in the Liver Tetraculture MPS.

Peer-Reviewed Validation

NANOSTACKS™-based liver models validated in peer-reviewed publications demonstrating human-relevant liver function and mechanistic hepatotoxicity responses.

Supported study capabilities

Stromal and vascular modulation of hepatotoxicity

Fibrosis-linked toxicity mechanisms

Mechanistic hepatotoxicity analysis

Chronic exposure studies

Microenvironment-driven drug response

Key Readouts & Outputs

Albumin

Urea

ATP / LDH viability

CYP450 activity

Imaging compatibility

Time-dependent toxicity

Practical Details

Cell typesPrimary human hepatocytes, stellate cells, endothelial cells

Plate format24-well

Culture durationAcute to long-term

FlowOptional; dynamic flow via external orbital or perfusion systems

Assay compatibilityBiochemical, imaging, molecular

Intended usersPharma, biotech, academic labs

Liver MPS model progression

Depending on your research question, you may need simpler or more complex cellular configurations. Our liver MPS portfolio includes monoculture, triculture, and tetraculture options.

Liver Monoculture MPS

Primary human hepatocytes—for early hepatotoxicity screening and metabolic baseline

Liver Tetraculture MPS

Hepatocytes, Kupffer cells, stellate cells, endothelial cells—for immune–hepatic crosstalk and chronic exposure

Use this model in your research

The Liver Triculture MPS can be applied within different experimental workflows depending on your team's internal capabilities, infrastructure, and study objectives.

Run In-House

Purchase Liver Triculture MPS products and operate them in your own laboratory with your team.

Fully Managed Studies

Revivocell operates Liver MPS for hepatotoxicity and ADME studies from design to data delivery.

Get the Liver Triculture MPS

Request a quote or read the peer-reviewed publication for technical validation details.

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