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Resources

Peer-reviewed publications, application notes, posters, and protocols supporting the science behind NANOSTACKS™ and CELLBLOKS®.

Featured Publication

Latest Peer-Reviewed Research

A New Microphysiological Platform to Study the Permeation of Neuroactive Agents Across Intestinal and Brain Barriers
Peer-Reviewed • Open Access2026

A New Microphysiological Platform to Study the Permeation of Neuroactive Agents Across Intestinal and Brain Barriers

Pavan B., Botti G., Dalpiaz A., Sbordoni R., Talari A. & Llabjani V.

Biofabrication

Validates CELLBLOKS® and NANOSTACKS™ as robust in vitro tools for studying neuroactive drug transport across intestinal, blood-brain, and blood-CSF barriers — demonstrating that eugenol crosses both BBB and BCSFB and triggers dopamine release in neuronal compartments.

Peer-Reviewed Publications

Multicellular Hepatic In Vitro Models Using NANOSTACKS™
In Vitro Models (Springer Nature)• 2025

Multicellular Hepatic In Vitro Models Using NANOSTACKS™

Talari A., Sbordoni R. et al.

NANOSTACKS™ liver models predict DILI using human-relevant, animal-free platforms for toxicity testing.

Effects Of Hyaluronic Acid On Three Different Cell Types Of The Periodontium
Biomolecules• 2025

Effects Of Hyaluronic Acid On Three Different Cell Types Of The Periodontium

Barbieri A., Pitzurra L., Loos B. & Jansen I.D.C.

Epithelial cells, osteoblasts, and periodontal ligament fibroblasts co-cultured with hyaluronic acid for studying periodontal regeneration.

Introducing CELLBLOKS®
In Vitro Model• 2022

Introducing CELLBLOKS®

Llabjani V. et al.

Introduced CELLBLOKS® plug-and-play organ-on-a-chip technology for building complex organotypic models.

Application Notes & Experimental Models

Practical demonstrations of NANOSTACKS™ across key research areas.

Use of MEA and AI to assess seizurogenic potential of compounds
Application Note

Use of MEA and AI to assess seizurogenic potential of compounds

Combined MEA recordings with AI to assess seizurogenic potential, improving neurotoxicity screening for drug development.

Development of an in vitro model of the human brain using NANOSTACKS™
Application Note

Development of an in vitro model of the human brain using NANOSTACKS™

Developed 3D brain models using NANOSTACKS™ to study neurological diseases and test neuroactive compounds.

Modeling the Liver-Brain Axis with NANOSTACKS™
Application Note

Modeling the Liver-Brain Axis with NANOSTACKS™

Modeled organ crosstalk between liver and brain to study systemic drug toxicity and multi-organ interactions.

Blog & Updates

Beyond Animal Testing: How New Approach Methodologies Are Reshaping Preclinical Drug Development
Blog

Beyond Animal Testing: How New Approach Methodologies Are Reshaping Preclinical Drug Development

The pharmaceutical and biotech industries are undergoing a fundamental transformation in preclinical drug development. Driven by regulatory momentum, scientific evidence, ethical imperatives, and technological breakthroughs, organizations worldwide are transitioning from traditional animal models to New Approach Methodologies (NAMs)—a collection of innovative, human-relevant technologies that promise faster, more predictive, and more ethical drug discovery.

Apr 21, 20268 min
High-complexity in vitro models for the toxicity assessment of antibody-drug conjugates
Blog

High-complexity in vitro models for the toxicity assessment of antibody-drug conjugates

Antibody-drug conjugates (ADCs) target cancer cells while reducing harm to healthy tissue. However, off-target toxicity remains a concern, demanding thorough evaluation. Read this blog post to explore the role of advanced in vitro models for toxicity screening of ADCs.

Mar 5, 20246 min
5 tips for making your own complex in vitro model
Blog

5 tips for making your own complex in vitro model

Planning to develop your own complex in vitro model (CIVM) for your next study? Discover 5 essential tips from our research team to help you design, optimize, and validate sophisticated cellular models that better mimic human biology.

Jan 14, 20235 min
From a Petri dish to an organ-on-a-chip: Is it a big jump?
Blog

From a Petri dish to an organ-on-a-chip: Is it a big jump?

Explore the evolution of cell culture from traditional 2D petri dishes to advanced organ-on-a-chip microfluidics, their limitations, and how Revivocell's CELLBLOKS® technology bridges the gap between conventional methods and cutting-edge approaches.

Jun 25, 202010 min

Science Guides

In-depth technical overviews of key drug safety topics and how NANOSTACKS™ addresses them.

Science Guide

Liver Toxicity Screening & DILI Testing

Why standard 2D hepatocyte models miss DILI, what makes a good liver model, and how multicellular Liver MPS improves predictive accuracy.

DILIHepatotoxicityLiver MPS
Science Guide

Neurotoxicity Screening & CNS Safety Testing

Why conventional viability assays miss functional CNS risk, MEA electrophysiology, and how Brain MPS captures structural and network-level neurotoxicity.

NeurotoxicityMEABrain MPS
Science Guide

Multi-Organ Toxicity Screening & Systemic Safety

Why single-organ models miss systemic toxicity, how organ–organ crosstalk drives secondary injury, and how integrated multi-organ platforms address this gap.

Systemic ToxicityOrgan CrosstalkMulti-Organ MPS
Science Guide

Connected Barrier Modeling

How multi-barrier MPS systems reveal drug transport across sequential tissue interfaces — from gut to liver to brain — and why isolated barrier assays fall short.

BBBADMEBarrier Permeability

Conference Posters

Presented at leading international scientific conferences.

Eugenol induced dopamine release from neuron-like PC12 cells
Pavan et. al., 2023

Eugenol induced dopamine release from neuron-like PC12 cells

Studied blood-brain barrier permeation of neuroactive agents to improve CNS drug delivery predictions.

CELLBLOKS® and NANOSTACKS™: novel platforms for toxicology research
SOT, Nashville, USA, 2023

CELLBLOKS® and NANOSTACKS™: novel platforms for toxicology research

Showcased innovative platforms advancing toxicology research by mimicking in-vivo conditions and barrier functions.

NANOSTACKS™: novel platforms for Head and Neck Cancer Research
Khurram et al., 2023

NANOSTACKS™: novel platforms for Head and Neck Cancer Research

Created 3D head and neck cancer models for testing therapeutic responses in physiologically relevant environments.

Protocols

Step-by-step guides for working with NANOSTACKS™ in your lab.

Hepatocyte Culture Protocol for NANOSTACKS™
Protocol

Hepatocyte Culture Protocol for NANOSTACKS™

Step-by-step guidance for culturing primary human hepatocytes on NANOSTACKS™ for liver toxicity studies.

Neuronal Differentiation Protocol for NANOSTACKS™
Protocol

Neuronal Differentiation Protocol for NANOSTACKS™

Comprehensive protocol for differentiating SH-SY5Y cells on NANOSTACKS™ for neurotoxicity and neuroscience research.

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