Liver Tetraculture MPS

Immune-competent multicellular human liver microphysiological system with hepatocytes, Kupffer cells, stellate cells, and endothelial cells for immune–hepatic crosstalk and mechanistic DILI studies

Platform Architecture

The Liver Tetraculture MPS is built on the modular NANOSTACKS™ platform, incorporating primary human hepatocytes, Kupffer cells, hepatic stellate cells, and liver endothelial cells in physically separated compartments for investigation of immune–hepatic crosstalk and inflammation-driven toxicity.

Unlike lower-complexity liver models, this immune-competent configuration maintains hepatocyte–immune–stromal–endothelial interactions to capture mechanistic pathways underlying immune-mediated liver injury and chronic inflammatory responses.

Each layer represents a discrete, permeable cellular compartment.

Supported study capabilities

Immune–hepatic crosstalk studies

Inflammation-driven liver injury

Fibrosis-associated signaling

Chronic and repeat-dose exposure

Mechanistic hepatotoxicity analysis

Immune activation assessment

Key Readouts & Outputs

Albumin

Urea

ATP / LDH viability

CYP450 activity

Cytokine release

Immune activation markers

Imaging compatibility

Time-dependent toxicity

Validated Performance

The Liver Tetraculture MPS demonstrates sustained hepatocyte metabolic function in the presence of immune cells, stellate cells, and endothelial cells, confirming baseline performance of an immune-competent multicellular configuration.

Representative validation data

Albumin secretion over time in Liver Tetraculture MPS

Representative albumin secretion over extended culture periods demonstrates sustained hepatocyte metabolic competence in the presence of immune and stromal cells, validating the baseline performance of the tetraculture configuration.

Key Model Capabilities

≥28-day functional stability
Sustained hepatocyte metabolic function
Immune–hepatocyte–stromal crosstalk
Enhanced inflammation-relevant responses

This model is optimized for immune–hepatic crosstalk and chronic exposure studies and represents a multicellular immune-competent configuration for mechanistic assessment of inflammation-driven liver injury.

Peer-Reviewed Validation

NANOSTACKS™-based liver models validated in peer-reviewed publications demonstrating immune-competent liver function and mechanistic hepatotoxicity responses.

Practical Details

Cell typesPrimary human hepatocytes, Kupffer cells, stellate cells, endothelial cells

Plate format24-well

Culture durationAcute to long-term

FlowOptional; dynamic flow via external orbital or perfusion systems

Assay compatibilityBiochemical, imaging, molecular

Intended usersPharma, biotech, academic labs

Liver MPS model progression

Depending on your research question, you may need simpler or more complex cellular configurations. Our liver MPS portfolio includes monoculture, triculture, and tetraculture options.

Liver Monoculture MPS

Primary human hepatocytes—for early hepatotoxicity screening and metabolic baseline

Liver Triculture MPS

Hepatocytes, stellate cells, endothelial cells—for stromal and vascular modulation studies

Use this model in your research

The Liver Tetraculture MPS can be applied within different experimental workflows depending on your team's internal capabilities, infrastructure, and study objectives.

Run In-House

Purchase Liver Tetraculture MPS products and operate them in your own laboratory with your team.

Fully Managed Studies

Revivocell operates Liver MPS for hepatotoxicity and ADME studies from design to data delivery.

Get the Liver Tetraculture MPS

Request a quote or read the peer-reviewed publication for technical validation details.

View standard NANOSTACKS™ bundles →