Liver Monoculture MPS

Primary human hepatocyte microphysiological system for early liver safety and metabolism studies

Platform Architecture

The Liver Monoculture MPS is built on the modular NANOSTACKS™ platform, incorporating primary human hepatocytes in a physically defined microenvironment optimized for physiologically relevant metabolism and toxicity assessment.

Unlike static 2D cultures or spheroids, this configuration maintains human hepatocyte function over extended culture durations, enabling reliable kinetic assessment of drug metabolism and clearance pathways.

Each layer represents a discrete, permeable cellular compartment.

Supported study capabilities

Early hepatotoxicity screening

Metabolic stability and clearance studies

CYP induction and inhibition

Dose–response assessment

Go / no-go compound triage

Key Readouts & Outputs

Albumin

Urea

ATP / LDH viability

CYP450 activity

Imaging compatibility

Time-dependent toxicity

Validated Performance

The Liver Monoculture MPS demonstrates stable and human-relevant hepatocyte function over extended culture durations, as assessed using established biochemical markers and functional endpoints routinely applied in liver safety and metabolism studies.

Representative validation data

Albumin secretion over time in Liver Monoculture MPS

Representative albumin secretion over extended culture periods demonstrates sustained hepatocyte metabolic competence and functional stability, supporting long-term maintenance of human-relevant liver function in vitro.

Key Model Capabilities

≥31-day functional stability
Sustained albumin and urea production
Enhanced CYP450 activity
Predictive DILI responses

This model is optimized for hepatocyte-intrinsic metabolism and toxicity assessment and does not capture immune-mediated or stromal interactions, which are addressed in higher-complexity liver MPS configurations.

Peer-Reviewed Validation

NANOSTACKS™-based liver models validated in peer-reviewed publications demonstrating human-relevant liver function and hepatotoxicity relevance.

Practical Details

Cell typePrimary human hepatocytes

Plate format24-well

Culture durationAcute to long-term

FlowOptional; dynamic flow via external orbital or perfusion systems

Assay compatibilityBiochemical, imaging, molecular

Intended usersPharma, biotech, academic labs

Liver MPS model progression

Depending on the biological complexity required, additional liver MPS configurations are available.

Liver Triculture MPS

Hepatocytes with supporting non-parenchymal cells for stromal and vascular modulation studies.

Liver Tetraculture MPS

Hepatocytes, Kupffer cells, stellate cells, and endothelial cells for immune–hepatic crosstalk and chronic exposure studies.

Use this model in your research

The Liver Monoculture MPS can be applied within different experimental workflows depending on your team's internal capabilities, infrastructure, and study objectives.

Run In-House

Purchase Liver Monoculture MPS products and operate them in your own laboratory with your team.

Fully Managed Studies

Revivocell operates Liver MPS for hepatotoxicity and ADME studies from design to data delivery.

Get the Liver Monoculture MPS

Request a quote or review the peer-reviewed publication for technical validation details.

View standard NANOSTACKS™ bundles →