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CELLBLOKS® 3D stack Liver-on-a-Chip model

This models contains 24 individual liver-chips per plate. Each chip consists of multiple liver cell types grown in layered scaffold blocks, arranged in close proximity in a 3D stacked format and is ready to be exposed to test drugs

Product details

  • 24 Chips per plate

    Removable CultureBlocks™; each chip and cell type can be analysed separately

  • Readily grown liver tissues

    Tri-culture chip model (layered arrangement)

    Human hepatocytes (HepG2) cells, Human endothelial HUVEC cells & Fibroblast cells grown in layered scaffold blocks

    *other cell type options on request

  • Open access

    Direct access to liver cell layers and compounds can be directly added to the model

  • Flow enabled

    Use with or without media flow circulation

    Optimised cell maintenance medium provided

The model

Benefits

  • Physiologically relevant

    Constitutes key liver cell types including hepatocytes fibroblasts and endothelial cells allowing cell-cell interactions

  • Liver functionality

    Enhanced hepatic relevance including increased albumin, ureas and Cytochrome P450 production compared to standard hepatocyte mon-cultures

  • Industry compliant

    The platform is designed in a standard SBS plate format compatible with standard imaging and readout equipment

  • Ready to use

    Use Liver-on-a-Chip model directly after arrival for up to 14 days allowing you to perform your studies straight away

Product specification sheet

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Media flow enabled via gravity-driven perfusion

Dynamic media flow around and through the cell-layers enabling continues supply of nutrients and oxygen to the cells replicating liver tissue microenvironment  

Liver function data

  • Imaging of cells through 3D stacked liver-chip layers

    Cells were imaged live on day 3 in stacked layers using epi-fluorescent microscopy (10x magnification). Labling: HUVEC with Cell Tracker (Green, CMFDA), HepG2 cells with Cell Tracker (Red, CMTPX) and NIN/3T3 cells with Hoechst stain. Cells can be easy imaged through layers by standard inverted microscope.

  • Metabolism in HepG2 hepatocytes

    CYP3A4 expression levels in monoculture hepatocytes vs. tri-culture stacked set-up in CELLBLOKS®. In HepG2 heptocyes CYP3A4 activity increased 3 times compared to HepG2 monocultures.

  • Metabolism in human upcyte® primary hepatocytes

    CYP3A4 expression levels in monoculture hepatocytes vs. tri-culture stacked set-up in CELLBLOKS®. In tri-culture model CYP3A4 in primary hepatocytes is enhanced by up to 3 times compared to monoculture hepatocytes over the 12 day of culture.

Powerful Drug Induced Liver Injury (DILI) prediction capability

CELLBLOKS® 3D stack Liver-on-a-Chip model clearly distinguishes between hepatotoxic vs. their no-toxic structural analogues drugs at clinically relevant concentrations

  • Clozapine vs. Olanzapine

    Acute drug exposure (24h) of the liver-on-a-chip model* clearly distinguishes between known hepatotoxin Clozapine and its structurally non-toxic analogous compound Olanzapine at clinically relevant concentrations. Clozapine is classed as Most-DILI-concern by FDA whereas Olanzapine as Less-DILI-Concern. Clozapine induced toxicity in cells can be detected at concentrations < 10uM over Human plasma Cmax levels.

    *CELLBLOKS® 3D stack Liver-on-a-Chip model: [HepG2 + HUVEC + NIH/3T3)

  • Troglitazone vs. Pioglitazone

    Acute exposure (24h) of the liver-on-a-chip model* clearly distinguishes between known hepatotoxin Troglitazone and its non-toxic structurally analogous Pioglitazone at clinically relevant concentrations. Troglitazone is classed as Most-DILI-concern by FDA whereas Pioglitazone is classed as Less-DILI-Concern. Troglitazone induced toxicity in cells can be detected at concentrations < 10uM over Human plasma Cmax levels.

    *CELLBLOKS® 3D stack Liver-on-a-Chip model: [HepG2 + HUVEC + NIH/3T3)

  • Trovafloxactin vs. Levofloxactin

    Acute drug exposure (24h) of the liver-on-a-chip model* clearly distinguishes between known hepatotoxin Trovafloxacin and its structurally analogous non-toxic Levofloxactin at clinically relevant concentrations. Trovafloxacin is classed by FDA as Most-DILI-concern whereas Levofloxactin as Less-DILI-Concern. Trovafloxactin induced toxicity in cells can be detected at concentrations < 10uM over Human plasma Cmax levels.

    *CELLBLOKS® 3D stack Liver-on-a-Chip model: [HepG2 + HUVEC + NIH/3T3)

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