Explore our wide range of resources, including insightful publications, comprehensive protocols, informative posters, and engaging instructional videos, designed to enhance and support your research needs with our technology.

Scientific Publications

Effects Of Hyaluronic Acid On Three Different Cell Types Of The Periodontium In A Novel Multi-Culture Cell Plate: An Exploratory Study

Alessio Barbieri  , Luciano Pitzurra , Bruno Loos and Ineke D. C. Jansen (Biomolecules 2025)

This study investigated the effects of hyaluronic acid (HA) on three key periodontal cell types—epithelial cells, osteoblasts, and periodontal ligament fibroblasts—using both single-culture and a novel multi-culture platform (CELLBLOKS®).  The multi-culture approach enabled cell–cell communication, better mimicking in vivo conditions.Key findings include:
• HA significantly enhanced epithelial cell proliferation, regardless of whether it was applied as a coating or dissolved in medium.
• Osteoblast proliferation and osteogenic gene expression (ALP, CD44) increased when in co-culture with other cell types, independent of HA.
• Periodontal ligament fibroblasts showed no significant response to HA or co-culture conditions
. • All cell types maintained high viability across all conditions.The study highlights the value of multi-culture systems in replicating physiological interactions and suggests that HA may aid epithelial regeneration, while intercellular communication is crucial for osteogenesis. Further studies are recommended to explore HA’s mechanistic role in periodontal regeneration.

Application Note

Application Note: Use of MEA and AI to assess seizurogenic potential of compounds

In this application note, NANOSTACKS™ was successfully integrated with Microelectrode Array (MEA) technology to develop an AI-powered neurotoxicity screening platform. This system enables high-throughput assessment of seizure liability by analyzing neuronal electrical activity in response to drug compounds.The study demonstrated the effectiveness of this approach by
:✔ Establishing a neuronal-astrocyte co-culture model using human iPSC-derived dopaminergic neurons and astrocytes.
✔ Recording neural activity using Axion BioSystems’ 24-well CytoView MEA Plates.
✔ Implementing an AI-driven analysis method to differentiate between seizurogenic and non-seizurogenic compounds based on real-time electrophysiological data
.✔ Assigning probability scores to predict the likelihood of seizure induction at various drug concentrations.
This proof-of-concept AI model successfully identified seizurogenic patterns, confirming the compatibility of NANOSTACKS™ with MEA-based functional readouts. These findings lay the groundwork for further refinement of AI-powered in vitro neurotoxicity screening, providing a reliable, standardized, and scalable alternative to traditional neurotoxicity testing.

Application Note

Application Note: Development of an in vitro model of the human brain using NANOSTACKS™

In this application note a novel in vitro model of the human brain, comprising an astrocytoma cell line in addition to undifferentiated or differentiated SH-SY5Y cells, was developed using NANOSTACKS™. This study revealed heightened neurotoxicity of the compound methylglyoxal on the brain model including undifferentiated SH-SY5Y, as compared to monocultures of undifferentiated SH-SY5Y. Furthermore, an assessment of the neurotoxicity of the drug clomipramine was conducted by performing a neurite outgrowth assay on the model including differentiated SH-SY5Y, demonstrating the compatibility of NANOSTACKS™ with imaging-based toxicity assays.

Application Note

Application Note: Modeling the Liver-Brain Axis with NANOSTACKS™

Explore the use of NANOSTACKS™ for the development of an in vitro model of the liver-brain axis using SH-SY5Y, differentiated HepaRG® cells and an astrocytoma cell line. In the first section, this application note describes the characterisation of differentiated HepaRG® on NANOSTACKS™, demonstrating their viability and the expression of the enzyme CYP3A4 for 9 days. Additionally, the application note details the execution of a neurotoxicity assay on the liver-brain axis model, using paclitaxel as a test compound. In particular, a 26.8% increase in sensitivity to neurotoxicity was associated to the liver-brain axis model as compared to SH-SY5Y monocultures.

Scientific Publications

Introducing CELLBLOKS®: a novel organ-on-a-chip platform allowing a plug-and-play approach towards building organotypic models

Llabjani et.al., 2022

This publication highlights the innovative features and capabilities of CELLBLOKS®, our advanced organ-on-a-chip platform. It demonstrates the plug-and-play approach of building organotypic models, enabling researchers to replicate complex organ systems in a controlled and customizable environment. This publication serves as a valuable resource for researchers interested in leveraging CELLBLOKS® technology for their own studies and advancements in the field of organ-on-a-chip research.

Poster 

Eugenol induced dopamine release from neuron-like PC12 cells after its permeation across the physiological brain barriers simulated in a new organ-on-a-chip platform

Pavan et. al., 2023

This study explores Eugenol's neuropharmacological properties using the CELLBLOKS® organ-on-a-chip platform, allowing the simulation of multiple physiological barriers within one system. The models include human cerebral microvascular endothelial cells (hCMEC/D3) representing the BBB, Human Retinal Pigment Epithelial (HRPE) cells as a BCSFB model, and Rat small Intestinal Epithelial cells (IEC-6) mimicking the GI barrier. The research examines Eugenol's ability to induce dopamine release in neuron-like PC12 cells grown in NANOSTACKS^TM inserts.The findings extend beyond neuropharmacology, offering insights into Eugenol's interactions with these barriers. This research may have broader therapeutic implications, with the potential to impact various strategies. Presented at the 73rd SIF National Congress, this study marks a significant step in understanding Eugenol's pharmacology and its passage through physiological barriers

Poster

CELLBLOKS® and NANOSTACKS^TM: novel platforms for toxicology research

SOT, Nashville, USA, 2023

This informative poster showcases the innovative platforms of CELLBLOKS® and NANOSTACKS™ for advancing toxicology research. It highlights the key features and advantages of these novel technologies, emphasizing their ability to mimic in-vivo conditions including barrier functions and provide more accurate and reliable toxicity assessments.

Application Note

Development of Accurate 3D Models of Head and Neck Cancer Using Revivocell's NANOSTACKS^TM Platform

Khurram et al.,2023; School of Clinical Dentistry at The University of Sheffield, United Kingdom

In the proof of concept study conducted by Prof. Ali Khurram and his team from the School of Clinical Dentistry at The University of Sheffield, United Kingdom, they utilized cancer cells from the primary site of head and neck cancer, cancer cells from the metastasis site from the same patient, and normal fibroblasts. By incorporating these cell types into Revivocell's NANOSTACKSTM platform, the researchers aimed to develop accurate 3D models that mimic the tumor microenvironment and enable a better understanding of head and neck cancer progression and metastasis. This preliminary work, published by Prof. Ali Khurram et al., highlights the potential of Revivocell's technology for advanced cancer research and sets the stage for further investigations into therapeutic interventions.

Video

Introduction to CELLBLOKS® Platform: Exploring the Basic Principles of Organ-on-a-Chip Technology

Duration: 1:50 

In this informative video, we introduce you to the CELLBLOKS® platform, a revolutionary technology enabling the development of organ-on-a-chip models. Learn about its key features, precise control of microenvironmental conditions, and its versatile applications in research. Watch the video to explore the exciting world of organ-on-a-chip technology with CELLBLOKS®

Video

Building Organ Models with CELLBLOKS® NANOSTACKST^TM Platform

Revivocell

In this video, we showcase an example of how the CELLBLOKS® NANOSTACKS® platform is used to construct a liver-on-a-chip model. The video demonstrates the step-by-step process of assembling the organ model using the modular components of the platform. It highlights the versatility of the platform, emphasizing that the same approach can be applied to create models of other single and multiple organ interactions. Watch the video to see how the CELLBLOKS® NANOSTACKS® platform revolutionizes the development of advanced organ models for various applications in the field of life sciences and drug discovery.

Blog

Human Organs-on-Chips: easier said than done

Dr. Valon Llabjani (CEO)

Discover the current state of the art in organ-on-a-chip technologies and how they compare to conventional cell culture methods. Dr. Valon Llabjani, Founder and CEO of Revivocell Limited, explores the advancements, limitations, and the need for improved models. Learn about Revivocell's CELLBLOKS® technology, a modular multi-organ/cell type co-culture system that bridges the gap between traditional methods and organs-on-a-chip approaches.

Blog

5 tips for making your own complex in vitro model

Dr. Raffaello Sbordoni (Research Scientist)

Discover 5 essential tips for designing and setting up a complex in vitro model (CIVM) that mimics the human body more effectively than traditional models. From maximizing cell types to validating your model, these insights will help you advance your research and increase its relevance

Flyers

Introducing CELLBLOKS® NANOSTACKS™: Unlocking New Frontiers in Organ-on-a-Chip Technology

Revivocell 

Discover the power of CELLBLOKS® NANOSTACKS™, the groundbreaking platform that transforms organ-on-a-chip technology. This flyer highlights the modular design, compatibility with diverse cell types, and ability to replicate complex organ interactions. Experience the future of biomedical research with CELLBLOKS® NANOSTACKS™, driving advancements in drug discovery, toxicity testing, and personalized medicine. Elevate your research today with CELLBLOKS® NANOSTACKS™.

Flyers

Introducing Standard CELLBLOKS® Platform: Empowering Organ-on-a-Chip Research

Revivocell 

 Explore the possibilities of organ-on-a-chip research with the Standard CELLBLOKS® Platform. This flyer showcases the versatility and convenience of the platform, offering standardized components for easy assembly and integration. With precise control over microenvironmental conditions, researchers can mimic the complexities of human organs and gain valuable insights into disease mechanisms, drug responses, and tissue engineering. Take your research to new heights with the Standard CELLBLOKS® Platform and unlock the potential of organ-on-a-chip technology.