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Multiorgan toxicity testing

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Why us

Our NANOSTACKSTM-based in vitro models recapitulate the cell-cell crosstalk and spatial cellular arrangement typical of the in vivo environment. This way, toxicity screening on our models can yield more in-vivo relevant results than the use of traditional models.

Liver-brain model: multiorgan toxicity

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The model is built on 3 layers, using our proprietary technology NANOSTACKS™

  • Layer 1: Liver component
  • Cell type: differentiated HepaRG
  • Differentiated HepaRG cells replicate functional characteristics of primary human hepatocytes such as CYP activity, and can therefore be used for metabolic and hepatotoxicity studies.
  • Layer 2: Glial component 
  • Cell type: U-138 MG
  • U-138 MG cells are an astrocytoma cell line used in the model to model a glial, astrocyte-like component.
  • Layer 3: Neuronal component
  • Cell type: SH-SY5Y
  • Handling: each model can be moved or disassembled into independent NANOSTACK™ with the use of sterile forceps.




Contact us for alternative endpoints.


Application Note

Application Note: Modeling the Liver-Brain Axis with NANOSTACKS™

This comprehensive note encompasses the characterization of differentiated HepaRG cells on NANOSTACKS™, providing insights into their behaviour and functionality. Additionally, it outlines the execution of neurotoxicity testing, shedding light on how NANOSTACKSTM facilitates the study of intercellular interactions and potential cross-effects between liver and brain cells.

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Unique Benefits

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Human relevant

All cells included in the model are of human origin

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Intercellular communication

Cell-cell crosstalk is replicated in the model, increasing its similarity to the in vivo environment

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Liver metabolism

HepaRG cells display CYP activity, and are therefore capable of metabolising compounds. This allows the system to also evaluate any neurotoxic effects of the compound’s metabolites

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Astrocytic support

The presence of astrocytes, which in vivo provide trophic support to neurons, is modeled by the inclusion of U-138 MG, increasing the neurobiological relevance of the model

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Differential toxicity evaluation

Toxicity can be evaluated on each individual cell type included in the model

Get in Touch

Explore how our models support your research studies

Alternative solutions

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Alternatively to performing our services within our facilities, we can also ship our validated organ models directly to you lab ready for immediate use.

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We can develop custom organ models using our NANOSTACKSTM 'Plug&Play' technology based on your requirements.

Need some help?

If you're interested in discovering how  our organ models can benefit your research, we invite you to get in touch with us. Our team is available to provide further insights and address any inquiries you may have.

Contact information


Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane Daresbury, Cheshire, WA4 4AD




BioLabs@NYU LANGONE, 180 Varick St, Floor 6, New York, NY 10014


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