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3D Stack Liver-Chip: HepG2 model
3D Stack Liver-Chip: HepG2 model
3D Stack Liver-Chip: HepG2 model
3D Stack Liver-Chip: HepG2 model
3D Stack Liver-Chip: HepG2 model
3D Stack Liver-Chip: HepG2 model

3D Stack Liver-Chip: HepG2 model

£0.00
Specifications 
Number of liver-chips per plate24 chips per plate. Each chip contains 3 cell layers
CellsHuman hepatocytes HepG2 cells (bottom layer) NIH/3T3 Fibroblast cells* (middle layer)
Human endothelial HUVEC cells (top layer)
* other cell types on request 
Organ platfromCELLBLOCKS® 3D layout Liver-on-a-Chip model
Membrane pore size0.4um
Membrane properties Transparent PET
Cell growth area0.28 cm2
Working Volume1ml

3D Stack HepG2 Liver-chip-model

Characterisation Data 

Enhanced Hepatic Function on 3D Stack Liver Model

Imaging of cells through 3D stacked liver-chip layers

Cells were imaged live on day 3 in stacked layers using epi-fluorescent microscopy (10x magnification). Labling: HUVEC with Cell Tracker (Green, CMFDA), HepG2 cells with Cell Tracker (Red, CMTPX) and NIN/3T3 cells with Hoechst stain. Cells can be easy imaged through layers by standard inverted microscope

Metabolism in HepG2 hepatocytes

CYP3A4 expression levels in monoculture hepatocytes vs. tri-culture stacked set-up in CELLBLOKS®. In HepG2 heptocyes CYP3A4 activity increased 3 times compared to HepG2 monocultures.

Metabolism in human upcyte® primary hepatocytes

CYP3A4 expression levels in monoculture hepatocytes vs. tri-culture stacked set-up in CELLBLOKS®. In tri-culture model CYP3A4 in primary hepatocytes is enhanced by up to 3 times compared to monoculture hepatocytes over the 12 day of culture

Powerful Drug Induced Liver Injury (DILI) prediction capability

CELLBLOKS® 3D stack Liver-on-a-Chip model clearly distinguishes between hepatotoxic vs. their no-toxic structural analogues drugs at clinically relevant concentrations

Hepatotoxicity evaluation (24 h exposure) in CELLBLOKS®  NANOSTACK™ based 3D liver model

Hepatotoxic drug-induced toxicity in HepG2 cells can be detected at concentrations < 10 µM over human plasma Cmax levels. The model correctly differentiated the hepatotoxic effects of compounds at high DILI concern (Clozapine, Troglitazone, Trovafloxacin) as opposed to their non-toxic drug analogues (respectively Olanzapine, Pioglitazone, Levofloxacin) at clinically relevant concentrations, therefore demonstrating a robust DILI prediction capability.

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If you're interested in discovering how CELLBLOKS® OOC can benefit your research, we invite you to get in touch with us. Our team is available to provide further insights and address any inquiries you may have.  Feel free to explore the following contact options:

UK: Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane Daresbury, Cheshire,  WA4 4AD

US: BioLabs@NYU LANGONE 180 Varick St, Floor 6, New York, NY 10014  

UK +44(0)7588015987

US: 1(0) 3479697346  

Info@revivoccell.com  

info@revivocell.com

US: 1(0) 3479697346