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THE TECHNOLOGY

CELLBLOKS ™, is a multi-organ modular “plug and play” co-culture technology. It serves the purpose of emulating the organ microenvironment in a standard in-vitro setting. It provides the feasibility of studying the assays in 2-D, 3-D cell growth condition, and in static or flow system, by using a standard perfusion rocker.

CELLBLOKS ™ designed to provide handling advantages to study co-culture, tri-culture and multi-organ communication in their experimental design without compromising the ease of use of standard well plate format for handling and analysis.




How does it work?

Using CELLBLOKS ™ different cell types can be grown on Barrier Pods or Circulatory Pods that emulate barrier organs or those in systematic circulation. Pods containing organ specific cells can then be connected to each other in variety of ways to simulate complex organ-organ interactions. For instance, Intestinal cell are grown on Barrier Pods, whereas Liver and lung cells in Circulatory PODs.

In this way you can quickly and easy set up an experiment in the lab to simulate complex body conditions, for example, to test how organs communicate to each other when they are exposed to a test drug.

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The Platform


Cell-cell communication studies made easy

CELLBLOKS ™ platform has dimensions of a standard Tissue Culture well plate. It has four sperate elongated channels with location for three separate Pods. Each channel is filled with media (3-5 ml) to allow the cell-cell communication between Pods.



The Cell Growth Pods

Achieve better representation of different Organtype communications

CELLBLOKS ™ Pods are of three different types.


Firstly, the Barrier Pods contain a selective permeable membrane on the bottom of the Pod, allowing cell to proliferative on basolateral membrane (simulating epithelial cells and tissues).


Secondly, the Circulatory Pods provide a flat plastic surface for cells to grow with side circulatory windows on the walls allowing selective media diffusion (both inlet and outlet, simulating organs in systematic circulation e.g. liver, brain, heart, lung).


Finally, the Blank Pods have the same flat surface as the Circulatory Pods for cell growth but no inlet or outlet for media diffusion. Blank Pods are used to isolate cell cultures from other compartments.







In addition, the modular nature of the device allows the removal of one or more cell growth Pods from the chamber to perform separate analysis without disturbing others cell compartments in culture.

Furthermore, as it provides separate compartments for each cells of a co-culture that cross-communicate among each other through media without leaving their compartment (unlike the conventional co-culture models), this permits user to study individual biology of each cell (e.g. protein, imaging, DNA).





Filtered Air Flow

Better ways to reduce contamination, media evaporation and avoid pH drifts

The CELLBLOKS ™chamber comes with new design Clip-Closure "Air-Lid" feature with two side clips that make it easy to create 100% hermetic seal around the chamber.

PTFE air filters and an o-ring seal have been incorporated in the lid in order to filter the air reaching cell cultures. Gas is in cultures is only exchanged through selectively permeable air filters (0.2 micorn) which reduce potential contamination of cells, evaporation of media, and fluctuations of pH during experimentation. In contrast to other cell culure platforms the Air-Lid technology is first-of-its-kind - enabling more reproducible data acqusitions and handling advantages.



Tri-organ co-culture example

The example below shows how an intestine-hepatic model can be simulated in the CELLBLOKS ™ platform to study the absorption, distribution and metabolism (ADME) of a drug or chemical as well as its effect on a target organ.

Briefly, the Intestinal layer is modelled by growing CaCo-2 cells, for example, on Barrier Pods that allows absorption of the drug. After absorption through the intestinal epithelium, the drug diffuses and is distributed into the channels (containing media) and to latter Pods containing seperate liver and lung compartments. The liver cells in the latter Circulatory Pod metabolises the drug and the third compartment (lung) is used as a target organ.



CELLBLOKS ™ can be used to set-up a tri-organ co-culture experiment and determine the effect of each compartment on the interaction between them. This is achieved in ONE PLATFOM by using combination of Barrier, Circulatory and Blank Pods. As illustrated below, 12 Pods are needed in total and they arranged in combinations of 3s, 2s and isolated blanks.





How do molecules diffuse betweeen Pods?
Watch the illustration below





Get a Quote

Chamber Chamber
  • Chamber & Air-Lid
  • Requires cell growth Pods (sold separately)
  • Catalog No: CBChamLid01
  • Pack of 1
  • 3-5 ml per channel
  • Fits 12 Pods
  • Email us
Chamber Chamber
  • Circulatory Pods
  • Catalog No: CBCirc01
  • Pack of 3
  • Membrane type: PC
  • Pore Size: 1 micron
  • Cell growth area: 2.9 cm2
  • Growth Medium: 1-2 ml
  • Email us
Chamber Chamber
  • Barrier Pods
  • Catalog No: CBBarrier01
  • Pack of 3
  • Membrane type: PC
  • Pore Size: 1 micron
  • Cell growth area: 1.2 cm2
  • Growth Medium: 1-2 ml
  • Email us
Chamber Chamber
  • Blank Pods
  • Catalog No:CBBlank01
  • Pack of 3
  • Membrane type: PC
  • Pore Size: 1 micron
  • Cell growth area: 2.9 cm2
  • Growth Medium: 1-2 ml
  • Email us





Contact Us



Address

Sci-Tech Daresbury, Keckwick Lane Daresbury Warrington, WA4 4AD

Phone Number

+44 (0) 1925864860