CELLBLOKS ™, is a multi-organ modular “plug and play” co-culture technology. It serves the purpose of emulating the organ microenvironment in a standard in-vitro setting. It provides the feasibility of studying the assays in 2-D, 3-D cell growth condition, and in static or flow system, by using a standard perfusion rocker.
CELLBLOKS ™ designed to provide handling advantages to study co-culture, tri-culture and multi-organ communication in their experimental design without compromising the ease of use of standard well plate format for handling and analysis.
How does it work?
Using CELLBLOKS ™ different cell types can be grown on Barrier Pods or Circulatory Pods that emulate barrier organs or those in systematic circulation. Pods containing organ specific cells can then be connected to each other in variety of ways to simulate complex organ-organ interactions. For instance, Intestinal cell are grown on Barrier Pods, whereas Liver and lung cells in Circulatory PODs.
In this way you can quickly and easy set up an experiment in the lab to simulate complex body conditions, for example, to test how organs communicate to each other when they are exposed to a test drug.
Cell-cell communication studies made easy
CELLBLOKS ™ platform has dimensions of a standard Tissue Culture well plate. It has four sperate elongated channels with location for three separate Pods. Each channel is filled with media (3-5 ml) to allow the cell-cell communication between Pods.
The Cell Growth Pods
Achieve better representation of different Organtype communications
CELLBLOKS ™ Pods are of three different types.
Firstly, the Barrier Pods contain a selective permeable membrane on the bottom of the Pod, allowing cell to proliferative on basolateral membrane (simulating epithelial cells and tissues).
Secondly, the Circulatory Pods provide a flat plastic surface for cells to grow with side circulatory windows on the walls allowing selective media diffusion (both inlet and outlet, simulating organs in systematic circulation e.g. liver, brain, heart, lung).
Finally, the Blank Pods have the same flat surface as the Circulatory Pods for cell growth but no inlet or outlet for media diffusion. Blank Pods are used to isolate cell cultures from other compartments.
In addition, the modular nature of the device allows the removal of one or more cell growth Pods from the chamber to perform separate analysis without disturbing others cell compartments in culture.
Furthermore, as it provides separate compartments for each cells of a co-culture that cross-communicate among each other through media without leaving their compartment (unlike the conventional co-culture models), this permits user to study individual biology of each cell (e.g. protein, imaging, DNA).
Filtered Air Flow
Better ways to reduce contamination, media evaoporation and avoid pH drifts
The CELLBLOKS ™chamber comes with new design Clip-Closure feature with two side clips that make it easy to create 100% hermetic seal around the chamber.
PTFE air filters have been incorporated on top of the lid in order to filter the air reaching cell cultures. This reduces potential contamination of cells, evaporation of media, and fluctuations of pH during experimentation. In contrast to conventional cell culture dishes this unique feature providing more reproducible data.
Tri-organ co-culture example
As illustrated in the example below an intestine-hepatic model can be simulated in the CELLBLOKS ™ platform to study the absorption, distribution and metabolism (ADME) of a drug or chemical as well as its effect on a target organ.
Briefly, the Intestinal layer is modelled by growing CaCo-2 cells, for example, on Barrier Pods that allows absorption of the drug. After absorption through the intestinal epithelium, the drug diffuses and distributed into the chamber (containing media) and to latter Pods containing sperate liver and lung compartments. The liver cells in the latter Circulatory Pod metabolises the drug and the third compartment (lung) is used as a target organ.
This illustration shows how CELLBLOKS ™ can be used to set-up a tri-organ co-culture experiment and determine the effect of each compartment on the interaction between them. This is achieved by using combination of Barrier, Circulatory and Blank Pods.
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